期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 5, 页码 2130-2134出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.09.020
关键词
Impedimetric immunosensor; Monoclonal antibody; Gold nanoparticles; Insulin like growth factor-1
类别
资金
- Isfahan University of Technology Council and Center of Excellency in Green Chemistry, Sensors and Nanotechnology in the Environment Research
An electrochemical impedimetric immunosensor was developed for ultrasensitive determination of insulin-like growth factor-1 (IGF-1) based on immobilization of a specific monoclonal antibody on gold nanoparticles (GNPs) modified gold electrode. Self-assembly of colloidal gold nanoparticles on the gold electrode was conducted through the thiol groups of 1,6-hexanedithiol (HDT) monolayer as a cross linker. The redox reactions of [Fe(CN)(6)](4-)/IFe(CN)(6)](3-) on the electrode surface was probed for studying the immobilization and determination processes, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The interaction of antigen with grafted antibody recognition layer was carried out by soaking the modified electrode into antigen solution at 37 degrees C for 3 h. The immunosensor showed linearity over 1.0-180.0 pg mL(-1) and the limit of detection was 0.15 pg mL(-1). The association constant between IGF-1 and immobilized antibody was calculated to be 9.17 x 10(11) M-1. The proposed method is a useful tool for screening picogram amounts of IGF-1 in clinical laboratory as a diagnostic test. (C) 2010 Elsevier B.V. All rights reserved.
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