期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 11, 页码 4320-4325出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.04.025
关键词
Silica sphere; Gold nanoparticle; Multiwall carbon nanotube; Nanocomposite biosensor; Methyl parathion hydrolase
类别
资金
- National Natural Science Foundation of China [20972056, 21075047]
- PCSIRT [IRT0953]
- Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission
- Ministry of Education, South-Central University for Nationalities
This article reports the fabrication of a nanocomposite biosensor for the sensitive and specific detection of methyl parathion. The nanocomposite sensing film was prepared via the formation of gold nanoparticles on silica particles, mixing with multiwall carbon nanotubes and subsequent covalent immobilization of methyl parathion hydrolase. The composite of the individual materials was finely tuned to offer the sensing film with high specific surface area and high conductivity. A significant synergistic effect of nanocomposites on the biosensor performance was observed in biosensing methyl parathion. The square wave voltammetric responses displayed well defined peaks, linearly proportional to the concentrations of methyl parathion in the range from 0.001 mu g mL(-1) to 5.0 mu g mL(-1) with a detection limit of 0.3 ng mL(-1). The application of this biosensor in the analysis of spiked garlic samples was also evaluated. The proposed protocol can be used as a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme-specific electroactive species. (C) 2011 Elsevier B.V. All rights reserved.
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