期刊
BIOSENSORS & BIOELECTRONICS
卷 28, 期 1, 页码 454-458出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.06.040
关键词
Label-free; Gold nanoparticles; Aptasensor; Electrochemistry; Aptamer; Thrombin
类别
资金
- Converging Research Center through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010K001120]
- Fundamental R&D Program for Core Technology of Materials
- Ministry of Knowledge Economy
- KRIBB Initiative (KRIBB, Korea)
This paper describes a novel approach to label-free electrochemical detection of human alpha-thrombin in human blood serum that utilizes ferrocene-coated gold nanoparticles (Fc-AuNPs). Human alpha-thrombin was specifically bound by the thiolated aptamers immobilized on the electrode. Positively charged Fc-AuNPs were electrostatically bound to the negatively charged aptamers. In principle, a high current peak should be observed in the absence of interactions between the aptamers and the human alpha-thrombin. This behavior indicates maximum adsorption of Fc-AuNPs by the negatively charged aptamers on the electrode surface. In contrast, when the thrombin-aptamer complex is formed, a low signal is expected because of the blocking capacities of the protein, which hinders the electrostatic binding of the Fc-AuNPs. The electrochemical signal, recorded by cyclic voltammetry and differential pulse voltammetry, indicates whether interactions between aptamers and proteins have occurred. There is a good correlation between the ferrocene oxidation peak intensity readings from our thrombin sensing system and the thrombin concentration, within the range of 1.2 mu M-12 mu M. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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