期刊
BIOSENSORS & BIOELECTRONICS
卷 27, 期 1, 页码 71-76出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.06.017
关键词
Biosensor; Immunosensor; Immunoassay; Carbon nanotube; Palladium nanoparticles; Screen-printed electrode
类别
资金
- National Basic Research Program of China [2010CB732400]
- National Science Fund for Creative Research Groups [20821063]
- National Natural Science Foundation of China [20875044, 21075055]
- Natural Science Foundation of Jiangsu [BK2008014]
A palladium nanoparticle decorated carbon nanotube was designed as a label for preparation of a highly sensitive disposable immunosensor. The immunosensor was constructed by assembling the capture antibody on gold nanoparticles decorated graphene nanosheets modified screen printed carbon working electrode. With a sandwich immunoassay mode, the palladium nanoparticle decorated carbon nanotubes were captured to the immunocomplex and showed strong electrocatalytic activity toward oxygen reduction. The use of carbon nanotube carrier offered a high amount of palladium nanoparticles on each immunoconjugate, hence amplified the detectable signal from the electro-reaction of dissolved oxygen. The graphene nanosheets and gold nanoparticles improved the electronic conductivity and the hydrophilicity of electrode surface for immobilization of the capture antibody, respectively. Under optimal conditions, a linear detection range from 50 pg/mL to 10 ng/mL and a limit of detection of 44 pg/mL (0.3 pM) were achieved for human IgG. Using dissolved oxygen as a signal reporter, the detection process avoided deoxygenation. The immunosensor showed acceptable stability, precision and accuracy, indicating potential applications in clinical diagnostics. (C) 2011 Elsevier B.V. All rights reserved.
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