期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 6, 页码 2953-2959出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.11.045
关键词
Three-step electrodeposition; Au microsphere; Self-doped polyaniline nanofiber; DNA hybridization recognition; Electrochemical impedance spectroscopy
类别
资金
- National Natural Science Foundation of China [20635020, 20805025, 20975057]
- Foundation of Qingdao City [09-1-3-25-jch]
- QUST [0022278]
A three-step electrodeposition method has been successfully adopted to fabricate morphology-controlled novel Au microspheres on self-doped polyaniline nanofibers (nanoSPAN) modified glassy carbon electrode. The deposition conditions, such as HAuCl4 concentration and deposition step, have significant influences on the morphologies and electrochemical properties of the resulted Au microspheres. Well hierarchical and homogeneously dispersed flower-like Au microspheres (HHFAu) were obtained under optimal conditions by the three-step electrodeposition strategy in 5.0 mM HAuCl4 solution. HHFAu possess large surface area, excellent electron transfer ability and good biocompatibility. The DNA probe could be effectively attached to HHFAu and thus a high-performance DNA biosensor was constructed by using electrochemical impedance spectroscopy as detection method. A gene fragment of the cauliflower mosaic virus 35S gene, which is related to one of the screening genes for the transgenically modified plants, has been satisfactorily detected. The linear range was from 1.0 x 10(-13) M to 1.0 X 10(-6)M and the detection limit was 1.9 x 10(-14) M. This HHFAu/nanoSPAN-based impedance biosensing platform holds great promise for the detection of other biological and chemical molecules. (C) 2010 Elsevier B.V. All rights reserved.
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