期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 9, 页码 3788-3793出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.02.033
关键词
Gold nanoparticle; ATRP; Biosensor; Immunoassay
类别
资金
- National Basic Research Program of China [2010CB732400]
- National Natural Science Foundation of China [20875013]
- Specialized Research Funds for the Doctoral Program of Higher Education [200802860035]
A novel colorimetric immunosensing strategy based on protein-modified gold nanoparticle probes combined with atom transfer radical polymerization (ATRP) technology was proposed. Gold nanoparticles (GNPs, similar to 15 nm) were functionalized with antibodies through an acylamide-bond between the carboxylic group of 11-mercaptoundecanoic acid that previously self-assembled on the surface of GNPs and the amino group of the protein (here, goat anti-rabbit immunoglobulim G (anti-IgG) used as model). The surface functionalized GNPs were used for IgG capture, which introduced initiator coupled anti-IgG (Ab2*) onto the surface of GNPs through immunoreactions. Subsequently triggered polymer growth resulted in the surface graft of preformed polymer chains onto nanoparticles that altered the optical property of GNPs. A distinct color change occurred. This could be designed for IgG detection. The spectrum absorption and colorimetric detection gave a linear range of 0.5-25 ng mL(-1) with a detection limit of 0.03 ng mL(-1) for IgG. The proposed approach showed high sensitivity from both visual and absorbance measurements. In spite of the limitations of available IgG antibodies, this approach could be easily extended to the detection of other biomarkers. (C) 2011 Elsevier B.V. All rights reserved.
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