期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 5, 页码 2311-2316出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.09.058
关键词
Chemically synthesized catecholamine; polymers; Glucose oxidase; Thermostability; Biosensing; Biofuel cell
类别
资金
- National Natural Science Foundation of China [20675029, 90713018, 20335020]
- State Special Scientific Project on Water Treatment [2009ZX07212-001-06]
- Foundations of Hunan Provincial Education Department [05K009]
- State Key Laboratory of Chemo/Biosensing and Chemometrics [200902]
Rapid oxidation of dopamine (DA) or L-noradrenaline (NA) by K(3)Fe(CN)(6) yields poly(DA) (PDA(C))or poly(NA) (PNA(C)) with glucose oxidase (GOx) effectively entrapped, and such an enzyme-entrapped catecholamine polymer is cast on an Au electrode followed by chitosan (CS) strengthening for biosensing and fabrication of a biofuel cell (BFC). The optimized glucose biosensor of CS/PDA(C)-GOx/Au displays an extremely high sensitivity up to 135 mu A mM(-1) cm(-2), a very low limit of detection of 0.07 mu M, a response time of <3 s, good suppression of interferents, striking thermostability (lifetime of 3 weeks at 60 degrees C and over 2 months at 30 degrees C), and high resistance to urea denaturation. The biosensor also works well in the second generation biosensing mode with p-benzoquinone (BQ) or ferrocene monocarboxylic acid (Fc) as an artificial mediator, with greatly broadened linear detection ranges (2.0 mu M-48.0 mM for BQ and 2.0 mu M-16.0 mM for Fc) and up to mA cm(-2)-scale glucose-saturated current density. The good permeability of artificial mediators across the enzyme film enables the quantification of the surface concentration of immobilized GOx on the basis of a reported kinetic model, and UV-Vis spectrophotometry is used to measure the enzymatic activity, revealing high enzymatic activity/load at CS/PDA(C)-GOx/Au. A BFC is also successfully fabricated with a bioanode of CS/PDA(C)-GOx/Au in phosphate buffer solution containing 100 mM glucose and 4.0 mM BQ and a carbon cathode in Nafion-membrane-isolated acidic KMnO(4), and its maximum power density of 1.62 mW cm(-2) is superior to those of most BFC hitherto reported. (C) 2010 Elsevier B.V. All rights reserved.
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