期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 5, 页码 1143-1148出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.09.044
关键词
Plant-tissue based biosensor; Amperometry; Peroxidase; Benzoyl peroxide
类别
资金
- Fundacao de Amparo A Pesquisa do Estado de Sao Paulo (FAPESP)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- IM2C
- CTPetro
- SAMUTI
This paper describes the applications of anew carbon paste electrode containing fibers of coconut (Cocus nucifera L) fruit, which are very rich in peroxidase enzymes naturally immobilized on its structure. The new sensor was applied for the amperometric quantification of benzoyl peroxide in facial creams and dermatological shampoos. The amperometric measurements were performed in 0.1 mol L-1 phosphate buffer (pH 5.2), at 0.0 V (versus Ag/AgCl). On these conditions, benzoyl peroxide was rapidly determined in the 5.0-55 mu mol L-1, with a detection limit of 2.5 mu mol L-1 (s/n = 3), response time of 4.1 s (90% of the steady state) and sensitivity limit of 0.33 A mol L-1 cm(-2). The amperometric results are in good agreement with those obtained by spectrophotometric technique, used as a standard method. (C) 2009 Elsevier B.V. All rights reserved.
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