期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 11, 页码 2503-2508出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.04.018
关键词
Polypyrrole-polyaniline copolymer; Carbon nanotubes; Organophosphates; Acetylcholinesterase; Biosensor
类别
资金
- National Natural Science Foundation of China [20705010]
- Research Fund for the Doctoral Program of Higher Education of China [20070511015]
A simple method to immobilize acetylcholinesterase (AChE) on polypyrrole (PPy) and polyaniline (PANI) copolymer doped with multi-walled carbon nanotubes (MWCNTs) was proposed. The synthesized PAn-PPy-MWCNTs copolymer presented a porous and homogeneous morphology which provided an ideal size to entrap enzyme molecules. Due to the biocompatible microenvironment provided by the copolymer network, the obtained composite was devised for AChE attachment, resulting in a stable AChE biosensor for screening of organophosphates (OPs) exposure. MWCNTs promoted electron-transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of OPs on the AChE activity, using malathion as a model compound, the inhibition of malathion was proportional to its concentration ranging from 0.01 to 0.5 mu g/mL and from 1 to 25 mu g/mL, with a detection limit of 1.0 ng/mL. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors. (C) 2010 Elsevier B.V. All rights reserved.
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