期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 7, 页码 1566-1570出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.10.013
关键词
Organaphosphorous hydrolase; Organophosphate pesticides; Carbon nanotube; Biosensor; Mesoporous carbon
To detect organophosphate chemicals, which are used both as pesticides and as nerve agents, a novel biosensor based on organo phosphorus hydrolase was developed. By using mesoporous carbon (MC) and carbon black (CB) as an anodic layer, the sensitivity of the sensor to p-nitrophenol (PNP), which is the product of the organophosphorus hydrolase reaction, was greatly improved. The MC/CB/glass carbon (GC) layer exhibited an enhanced amperometric response relative to a carbon nanotube (CNT)-modified electrode because it promoted electron transfer of enzymatically generated phenolic compounds (p-nitrophenol). The well-ordered nanopores, many edge-plane-like defective sites (EDSs), and high surface area of the MC resulted in increased sensitivity, and allowed for nanomolar-range detection of the analyte paraoxon. Thus, MCs are suitable for use in real-time biosensors. Under the optimized experimental conditions, the biosensor had a detection limit of 0.12 mu M (36 ppb) and a sensitivity of 198 nA/mu M for paraoxon. (C) 2009 Elsevier B.V. All rights reserved.
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