期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 3, 页码 1036-1042出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.08.047
关键词
Osteoarthritis; Synovial fluid; Interleukin-1 beta (IL-1 beta); Fiber-optic particle plasmon resonance; Gold nanoparticle; Self-assembled monolayer
类别
资金
- Buddhist Dalin Tzu Chi General Hospital [95-1-3 and 96-1-3]
- National Science Council (Republic of China) [NSC95-3114-P-194-001-MY3]
A facile and label-free biosensing method has been developed for determining an osteoarthritis concerned cytokine, interleukin-1 beta (IL-beta), in synovial fluids. The biosensing technique, fiber-optic particle plasmon resonance (FOPPR), is based on gold nanoparticles-modified optical fiber where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for further conjugation of anti-IL-1 beta antibody and minimization of nonspecific adsorption. Upon binding of IL-1 beta to anti-IL-1 beta on the gold nanoparticle surface, the absorbance of the gold nanoparticle layer on the optical fiber changes and the signal change is enhanced through multiple total internal reflections along the optical fiber. Results show that the detection of IL-1 beta in synovial fluid by this sensor agrees quantitatively with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method but a much shorter analysis time is required (<10 min). The sensor response versus log concentration of IL-1 beta was linear (r = 0.9947) over the concentration range of 0.050-10 ng/mL and a limit of detection (LOD) of 21 pg/mL (1.2 pM) was achieved. Such a LOD for IL-1 beta (17 kDa) represents a major advancement in the field of real-time monitoring of low molecular weight proteins in complex biological fluids. (C) 2010 Elsevier B.V. All rights reserved.
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