Preparation and application of magnetic reversed-phase restricted access material

Using magnetic silica (Fe2O3 @ SiO2) as the matrix material, a magnetic reversed-phase restricted access material was prepared via surface-initiation atom transfer radical polymerization (SI-ATRP) on the surface of modified Fe2O3 @ SiO2. Stearyl methacrylate (SMA) was grafted on the inner surface of the modified Fe2O3 @ SiO2, and glycidylmethacrylate (GMA) was grafted on the outer surface of the modified Fe2O3 @ SiO2. The magnetic reversed-phase restricted access material was prepared via acid hydrolysis. This material was characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and elemental analysis. The exclusion ability of the opened magnetic reversed-phase restricted access material to bovine serum albumin (BSA) was 90. 4%. The maximum adsorbed amounts of sulfisoxazole (SIZ), sulfadime-thoxine (SDM), trimethoprim (TMP) and sulfamerazine (SMR) were 2. 76 , 2. 24 , 1. 51 and 1. 34 mg/g, respectively. This material was applied to extract and enrich SIZ, SMR and SDM in milk and bovine serum samples. The spiked recoveries of SIZ, SMR and SDM were 88. 7% - 90. 8%, and the relative standard deviations were 3. 3% - 5. 3%. Thus, the magnetic phase restricted access material can simplify the pretreatment of biological matrix samples, and can be applied to the analysis and detection of blood samples or food samples.