期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 2, 页码 575-579出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.07.034
关键词
Aptamer; Gold nanoparticles; Amplification; QCM-D biosensor; Human alpha-thrombin
类别
资金
- National Natural Science Foundation of China [20975006, 90713013, 20675003]
- Ministry of Science and Technology of China [2007CB511903]
- State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University [2008004]
A highly sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor for protein was developed using aptamer-functionalized gold nanoparticles (Apt-GNPs) for amplification. Human alpha-thrombin, an important physiological protease found in blood, was chosen as the target protein. Captured by immobilized aptamers, thrombin was determined on-line using Apt-GNPs to enhance both frequency and dissipation signals. The fabricated sandwich of aptamer/thrombin/Apt-GNPs on chip surface was confirmed by atomic force microscopy (AFM). Compared to direct assay, the detection limit for thrombin was down to 0.1 nM, yielding about 2 orders of magnitude improvement in sensitivity. This aptamer-based QCM-D biosensor also showed good selectivity and repeatability in complex matrix. For the first time, the dual-signal enhancement of Apt-GNPs on QCM-D sensing was demonstrated, and such design could provide a promising detection strategy for proteins with two binding sites. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据