期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 5, 页码 1014-1018出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.09.019
关键词
Bienzymic biosensor; Ethanol and glucose detection; Self-assembled monolayer; Tetrathiafulvalene
类别
资金
- Ege University Research Fund [2008 FEN 014]
The aim of this project was to develop a bienzymic biosensor, which was based on co-immobilization of alcohol oxidase and glucose oxidase on the same electrode by formation of self-assembled monolayer (SAM) for selective determination of ethanol and glucose. In the biosensor construction the enzymes and the mediator, tetrathiafulvalene (TTF), were immobilized with cross-linking agents glutaraldehyde and cysteamine by forming a self-assembled monolayer (SAM) on a gold disc electrode. Amounts of ethanol and glucose were amperometrically detected by monitoring current values at reduction potential of TTF+, 0.1 V. Decreases in biosensor responses were linearly related to glucose concentrations between 0.1 and 1.0 mM and ethanol concentrations between 1.0 and 10 mM. Limits of detection of the biosensor for ethanol and glucose were calculated to be 0.75 and 0.03 mM, respectively. In the optimization studies of the biosensor some parameters such as optimum pH, optimum temperature, enzyme amount, effect of TTF concentration and duration of SAM formation were investigated. (C) 2009 Elsevier B.V. All rights reserved.
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