期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 10, 页码 2218-2224出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.02.023
关键词
Molecularly imprinted polymers; Mixed-templates; Tetracycline antibiotics; Molecular recognition ability; Foodstuffs
类别
资金
- National Nature Science Foundation of China [30771807, 20407009]
- National Basic research Grant (973) of China [2009CB320301]
- Nature Science Foundation of Hubei province [2008CDB175]
We developed in the present study a set of molecularly imprinted polymers (MIPs) by precipitation polymerization, using four members of tetracycline antibiotics (TCs) as templates (Oxytetracycline, Tetracycline, Chlortetracycline and Doxycycline). Then, based on the imprinting effect of different MIPs (TCs), Oxytetracycline and Chlortetracycline were chosen as mixed-templates to synthesize high selective MIPs for recognition of a family of analytes. And the characterization of obtained MIPs was analyzed by FESEM analysis, Brunauer-Emmett-Teller (BET) method, polymer size analysis, and determination of swelling ratio and COOH capacity. It was shown that the optimal imprinting factors of TCs on mixed-templates MIPs were more than 6.0 and the maximum binding amount of TCs were about 27 mu mol g(-1) (Oxytetracycline), 35 mu mol g(-1) (Tetracycline), 35 mu mol g(-1) (Chlortetracycline) and 39 mu mol g(-1) (Doxycycline), respectively. Finally, the mixed-templates MIPs were shown to be promising for on-line solid-phase extraction-HPLC-UV determination of trace TCs in foodstuffs. With a sample loading volume of 10 mL, the enhancement factors were in the range of 159-410. (C) 2010 Elsevier B.V. All rights reserved.
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