期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 6, 页码 1313-1318出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.10.018
关键词
Direct electrochemistry; Horseradish peroxidase; Nanowire array electrode; Self-assembled monolayer
类别
资金
- Enterprise Ireland (EI) [CFTD/2008/322]
- Irish Research Council for Science, Engineering and Technology (IRCSET), Ireland
Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H2O2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5 h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE(5h) (5 h deposition time) exhibited remarkable sensitivity (45.86 mu A mM(-1) cm(-2)) towards H2O2 with a detection limit of 0.42 mu M (S/N=3), linearity up to 15 mM and a response time of 4 s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors. (C) 2009 Elsevier B.V. All rights reserved.
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