期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 10, 页码 2309-2312出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.03.021
关键词
Polypyrrole; Nanowire; Spore detection; Bacillus sp.; Biosensor; Bioterrorism
类别
资金
- Ministry of Science and Innovation of Spain [CBET-0617240]
- United States NSF
- Consejo Superior de Investigaciones Cientificas (CSIC, Spain)
Polypyrrole nanowires (Ppy) were assembled onto microfabricated gold interdigitated microelectrodes, to construct a chemiresistive biosensor for the detection of Bacillus globigii, used as simulant of the threatening bioterrorism agent B. anthracis. The fabricated biosensor showed good linear correlation (r(2) = 0.992) for low spore concentrations ranging from 1 to 100 CFU (colony forming units)/mL, a concentration that could be used in a bioterrorism attack, with a response time of 30 min, after which the sensor was saturated. The performance of the biosensor was also assessed in the absence of anti-B. globigii antibodies and in the presence of non-target bacterial cells (Escherichia coli) showing no significant non-specific interactions. We believe that Ppy nanowires are a good platform for the detection and also quantification of large molecules and biocomponents even at low concentrations. (C) 2010 Elsevier B.V. All rights reserved.
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