期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 7, 页码 1658-1662出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.12.004
关键词
Electrochemical immunosensor; Sensor array; Hepatitis virus; Simultaneous determination
类别
资金
- National Basic Research Program of China [2010CB732403]
- National Natural Science Foundation of China [20877019, 20735002]
- Key Natural Sciences Foundation of Fujian Province, China [D0520001]
- Key Program of Science and Technology Department of Fujian Province, China [2007Y0026]
- NTU-MOE Academic Research Funds [RG65/08]
An integrated automatic electrochemical immunosensor array has been designed for the simultaneous detection of 5-type hepatitis virus antigens (i.e. hepatitis A, hepatitis B, hepatitis C, hepatitis D, and hepatitis E). Initially, 5-type hepatitis virus antibodies were immobilized onto a self-made electrochemical sensor array using nanogold particles and protein A as matrices, and the immunosensor array was then used to capture their corresponding antigens from sample solution with a 1-step capture format. The detection is based on the potential change before and after the antigen-antibody reaction by using a 2-electrode system. The developed immunosensor array with allowed simultaneous determination of 5-type hepatitis virus antigens in 5 min. The detection limit of the sensor array was <= 1.0 ng/mL for most analytes. The intra- and inter-assay imprecision (CVs) of the sensor array were <= 7.3% and 8.1%, respectively. No nonspecific adsorption was evaluated, and the crossing talk between neighboring sites was <= 7.5% (CV). The developed immunoassay system offers promise for label-free, simple, rapid, cost-effective analysis of multi-analyte. Importantly, the chip-based immunosensor array could open new opportunities for high-throughput multi-analyte immunoassays in point-of-care testing of miniaturized lab-on-a-chip devices. (C) 2009 Elsevier B.V. All rights reserved.
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