期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 6, 页码 1389-1394出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.10.034
关键词
Array; Biosensor; EIS; Gold; DNA; Aptamer
类别
资金
- US Army [W911SR-04-C-0061, W911NF-05-C-0019]
Using electrochemical impedance spectroscopy (EIS) for biosensing applications typically requires repetitive experiments. To address this need, we have designed a multispecific electrochemical array with eight individually addressable 2 mm-diameter gold working electrodes for rapid biosensing data accumulation by EIS in the presence of redox agent. The array allows to incorporate multiple negative controls in the course of a single binding experiment, as well as to perform parallel identical experiments to improve reliability of detection. The array is fit with attached electrochemical cell with Ag/AgCl mini reference electrode and can be used to process macro samples of 0.5-1 ml or micro samples of 5 mu l in a drop-wise fashion. Eight individual EIS measurements are completed in 15 min. The reported array is disposable, economical and is easy to use. Examples of array use for label-free genetic sensing of 2.7 kb-long target Yersinia pestis DNA and for protein sensing of Ricin Toxin Chain A (RTA) are presented. We suggest the reported array design as a tool for researchers in the area of EIS sensing. (C) 2009 Elsevier B.V. All rights reserved.
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