期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 3, 页码 1028-1035出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.08.045
关键词
Biochip; C-reactive protein (CRP); Label-free detection; Molecular switching fluorescence; Total internal reflection fluorescence; microscopy (TIRFM)
类别
资金
- Korea Ministry of Science and Technology [M1053608003-05N3608-00310]
- Korea Science & Engineering Foundation [R01-2007-000-20238-0]
A novel detection technique on biochip for the quantification of label-free C-reactive protein (CRP) based on molecular switching of fluorescence (MSF) is demonstrated by total internal reflection fluorescence microscopy. It alters fluorescence intensity of fluoreseinamine isomer 1 (FAI) upon binding with its specific ligand,O-phosphorylethanolamine (PEA). In the MSF-based detection, FAI was used as an ink, printed on a 3-glycidoxypropyl-trimethoxysilane (GPTS)-coated glass coverslip. With the addition of GPTS conjugated PEA solution to the FAI-printed coverslip, the fluorescence intensity was remarkably decreased. Addition of CRP increased fluorescence intensity linearly in the range of 800 aM to 500 fM (R = 0.997). The MSF-based biochip assay for the estimation of CRP in human sera showed similar to 200 times increased detection sensitivity in less than a third of the time to obtain results using a conventional enzyme-linked immunosorbent assay. This biochip detection is a promising new technique for the quantification of CRP molecules from trace amounts of clinical samples. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据