期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 3, 页码 1109-1113出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.08.021
关键词
Electrochemical immunoassay; Quantum dots; Biomarker; Phosphorylated bovine serum albumin
类别
资金
- Office of the Higher Education Commission, Thailand
- Thai Government
- National Institutes of Health through the National Institute of Neurological Disorders and Stroke, National Institute of Health [U01 NS058161-01]
- Department of Energy's Office of Biological and Environmental Research located at Pacific Northwest National Laboratory
- DOE [DE-AC05-76L01830]
- [PCU028.2010]
A CdSe/ZnS quantum dot (QD) based electrochemical immunoassay of phosphorylated bovine serum albumin (BSA-OP) as a protein biomarker is presented. The QDs were used as labels for amplifying electrochemical signals and were conjugated with a secondary anti-phosphoserine antibody in a heterogeneous sandwich immunoassay. In this assay, the model phosphorylated protein BSA-OP was added to the primary BSA antibody coated polystyrene microwells, and then the QD labeled anti-phosphoserine antibody was added for completing immunorecognition. Finally, the bound QD was dissolved in an acid-dissolution step and was detected by electrochemical stripping analysis. The measured current responses were proportional to the concentration of BSA-OP. Under optimal conditions, the voltammetric response was linear over the range of 0.5-500 ng mL(-1) of BSA-OP, with a detection limit of 0.5 ng mL(-1). It also shows good reproducibility with a relative standard deviation of 8.6%. This QD-based electrochemical immunoassay offers great promise for simple and cost-effective analysis of protein biomarkers. (C) 2010 Elsevier B.V. All rights reserved.
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