期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 6, 页码 1504-1508出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.11.009
关键词
Graphene; Ionic liquids; NADH; Electrocatalysis; Ethanol
类别
资金
- NSFC, China [20673109, 20827004]
- Ministry of Science and Technology [2007AA03Z354, 2007BAK261306]
It is firstly reported that low-potential NADH detection and biosensing for ethanol are achieved at an ionic liquid-functionalized graphene (IL-graphene) modified electrode. A substantial decrease (440 mV) in the overvoltage of the NADH oxidation was observed using IL-graphene/chitosan coating, with oxidation starting at ca. 0V(vs. Ag vertical bar AgCl). And the NADH amperometric response at such a modified electrode is more stable (95.4% and 90% of the initial activity remaining after 10 min and 30 min at 1 mM NADH solution) than that at bare electrode (68% and 46%). Furthermore, the IL-graphene/chitosan-modified electrode exhibited a good linearity from 0.25 to 2 mM and high sensitivity of 37.43 mu A mM(-1) cm(-2). The ability of IL-graphene to promote the electron transfer between NADH and the electrode exhibited a novel and promising biocompatible platform for development of dehydrogenase-based amperometric biosensors. With alcohol dehydrogenase (ADH) as a model, the ADH/IL-graphene/chitosan-modified electrode was constructed through a simple casting method. The resulting biosensor showed rapid and highly sensitive amperometric response to ethanol with a low detection limit (5 mu M). Moreover, the proposed biosensor has been used to determine ethanol in real samples and the results were in good agreement with those certified by the supplier. (c) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据