期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 12, 页码 2675-2679出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.04.045
关键词
Biosensor; Glucose oxidase; Carbon ionic liquid electrode; Colloidal gold; Acid stability
类别
资金
- National Outstanding Youth Foundations of China
- National Science Foundation of China [50725825]
- Special Research Found for the Doctoral Program of Higher Education of China [20060532006]
A colloidal gold-modified carbon ionic liquid electrode was constructed by mixing colloidal gold-modified graphite powder with a solid room temperature ionic liquid n-octyl-pyridinium hexafluorophosphate (OPPF6). Glucose oxidase (GOD) was entrapped in this composite matrix and maintained its bioactivity well and displayed excellent stability. The effect conditions of pH, applied potential and GOD loading were examined. Especially, the glucose oxidase entrapped in this carbon ionic liquid electrode fully retained its activity upon stressing in strongly acidic conditions (pH 2.0) for over one hour. The proposed biosensor responds to glucose linearly over concentration range of 5.0 x 10(-6) to 1.2 x 10(-3) and 2.6 x 10(-3) to 1.3 x 10(-2) M, and the detection limit is 3.5 x 10(-6) M. The response time of the biosensor is fast (within 10 s), and the life time is over two months. The effects of electroactive interferents, such as ascorbic acid, uric acid, can be significantly reduced by a Nafion film casting on the surface of resulting biosensor. (C) 2010 Elsevier B.V. All rights reserved.
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