期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 11, 页码 2539-2542出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.04.007
关键词
Amplification; Aptamers; Bi-enzyme; Biosensors; Proteins; Ultrasensitive detection
类别
资金
- National Natural Science Foundation of China [20905062, 20675064]
- Natural Science Foundation Project of Chongqing City [CSTC-2009BB5003]
- China Post-doctoral Science Foundation [20090460715]
- Southwest University [SWUB2008078, XDJK2009B013]
- US NIH [R01 EB002189, U01 AI075565]
We present an ultrasensitive aptasensor for the electronic monitoring of proteins through a dual amplified strategy in this paper. The target protein thrombin is sandwiched between an electrode surface confined aptamer and an aptamer-enzyme-carbon nanotube bioconjugate. The analytical signal amplification is achieved by coupling the signal amplification nature of multiple enzymes with the biocatalytic signal enhancement of redox-recycling. Our novel dramatic signal amplification strategy, with a detection limit of 8.3 fM, shows about 4 orders of magnitude improvement in the sensitivity for thrombin detection compared to other universal single enzyme-based assay. This makes our approach an attractive alternative to other common PCR-based signal amplification in ultralow level of protein detection. (C) 2010 Elsevier B.V. All rights reserved.
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