期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 3, 页码 615-622出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.01.027
关键词
Surface imprinting; Hydrogel; Bovine serum albumin; Silica microsphere
类别
资金
- National Natural Science Foundation of China [90717002, 30872109]
- National 863 Program [2006AA10Z447]
Surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. In this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble UV sensitive initiator onto the surface of silica beads. The polymerization was initiated by UV radiation with N-[3-(dimethylamino)propyl]methacrylamide and N-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat Of stimuli-responsive hydrogel yielded over the silica gels. The surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (K-a) of 2.2 x 10(5) Lmol(-1) and a maximum binding capacity (Q(max)) of 27.3 mg g(-1) in Tris-HCl buffer (pH 7.0). The rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3 h for the previously prepared bulk imprinted hydrogel). Besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. The above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials. (C) 2009 Elsevier B.V. All rights reserved.
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