4.8 Article

Molecularly imprinted nanopatterns for the recognition of biological warfare agent ricin

期刊

BIOSENSORS & BIOELECTRONICS
卷 25, 期 3, 页码 592-598

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.03.041

关键词

MIP; BWA; Ricin; Nanopatterns; Nanopores; Protein

向作者/读者索取更多资源

Molecularly imprinted polymer (MIP) for biological warfare agent (BWA) ricin was synthesized using silanes in order to avoid harsh environments during the synthesis of MIR The synthesized MIP was utilize for the recognition of ricin. The complete removal of ricin from polymer was confirmed by fluorescene spectrometer and SEM-EDAX SEM and EDAX studies confirmed the attachment of silane polymer on the surface of silica gel matrix. SEM image of Ricin-MIP exhibited nanopatterns and it was found to be entire different from the SEM image of non-imprinted polymer (NIP). BET surface area analysis revealed me surface area (227 m(2)/g) for Ricin-MIP than that of NIP (143 m(2)/g). In addition, surface area study all showed more pore volume (0.5010 cm(3)/g) for Ricin-MIP than that of NIP (0.2828 cm(3)/g) at 12 nm pore diameter confirming the presence of imprinted sites for ricin as the reported diameter of ricin is 12 The recognition and rebinding ability of the Ricin-MIP was tested in aqueous solution. Ricin-MIP reboue, more ricin when compared to the NIP. Chromatogram obtained with Ricin-MIP exhibited two peaks do, to imprinting, however, chromatogrant of NIP exhibited only one peak for free ricin. SDS-PAGE rest confirmed the second peak observed in chromatogram of Ricin-MIP as ricin peak. Ricin-MIP exhibited an imprinting efficiency of 1.76 and it also showed 10% interference from the structurally similar prote abrin. (C) 2009 Elsevier B.V. All rights reserver:

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据