期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 6, 页码 1621-1628出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.08.029
关键词
Bienzyme; Biocatalysis; Multiwalled carbon nanotubes; Polyaniline; Biosensor
类别
资金
- National Natural Science Foundation of China [20675062, 20875076]
- Research Fund for the Doctoral Program of Higher Education [20060697013]
A novel method based on covalent attachment of two enzymes, glucose oxidase (GOD) and horseradish peroxide (HRP), onto carboxylic-derived multiwalled carbon nanotubes (MWNTs) for the deposition of electroactive polyaniline (PANI) under ambient conditions is described. Ultraviolet-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy were used to characterize the assembling of bienzyme and the morphology of PANI vertical bar MWNTs. Under the bienzyme biocatalytic condition, a head-to-tail structure of PANI templated by MWNTs was formed. The voltammetric characteristics of the resulting biosensor were investigated by cyclic voltammetry in the presence of glucose. The current response of PANI was linearly related to glucose concentration between 0.05 and 12.0 mM with a correlation coefficient of 0.994. The synergistic performance of bienzyme, highly efficient polymerization, and templated deposition provide a general platform for the synthesis of nanowires and nanocircuits, the construction of bioelectronic devices, and the design of novel biosensors. (C) 2008 Elsevier B.V. All rights reserved.
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