期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 5, 页码 1522-1526出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.08.023
关键词
Photon upconversion; Sickle cell disease; Oligonucleotide detection; Point mutation; Locked nucleic acid
类别
资金
- NCRR NIH HHS [P20 RR016480-03, RR-016480, P20 RR016480] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016480] Funding Source: NIH RePORTER
We report the design of an oligonucleotide sensor for the detection of point mutation associated with sickle cell disease. The sensor was based on luminescence resonance energy transfer between a donor and an acceptor. Photon upconverting nanoparticles (NaYF4 doped with Yb3+ and Er3+) were used as the donor and a conventional fluorophore, N,N,N',N'-tetramethyl-6-carboxyrhodamine (TAMRA), as the acceptor. The sensor could detect the perfectly matched target, in the background of the mismatched target or other oligonucleotides of random sequences. The detection limit of this sensor towards perfectly matched target was calculated to be 120 femtomoles, with no photobleaching. Oligonucleotide sensors of such design demonstrate high sensitivity and specificity. (c) 2008 Elsevier B.V. All rights reserved.
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