期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 11, 页码 3372-3378出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.04.048
关键词
Carbon nanotube; Field effect transistor; Biosensor; Surface modification; Spacer; Prostate cancer
类别
资金
- Ministry of Commerce, Industry and Energy (MOCIE) [10017190]
- Korea government (MEST) [R0A-2008-000-20078-0, R0A-2004-000-10438-0]
- National Research Foundation of Korea [R0A-2004-000-10438-0, 과C6A1904] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This paper presents a simple and sensitive method for the real-time detection of a prostate cancer marker (PSA-ACT complex) through label-free protein biosensors based on a carbon nanotube field effect transistor (CNT-FET). Herein, the CNT-FET was functionalized with a solution containing various linker-to-spacer ratios, the binding event of the target PSA-ACT complex onto the receptor detected by monitoring the gating effect caused by charges in the target PSA-ACT complex. Since the biosensors were used in a buffer solution, it was crucial to control the distance between the receptors through introduction of linkers and spacers so that the charged target PSA-ACT complex could easily approach the CNT surface within the Debye length to give a large gating effect. The results show that CNT-FET biosensors modified with only linkers could not detect target proteins unless a very high concentration of the PSA-ACT complex solution (similar to 500 ng/ml) was injected, while those modified with a 1:3 ratio of linker-to-spacer could detect 1.0 ng/ml without any pretreatment. Moreover, our linker and spacer-modified CNT-FET could successfully block non-target proteins and selectively detect the target protein in human serum. Significantly, this strategy can be applied to general antibody-based detection schemes and enables production of very simple and sensitive electronic biosensors to detect clinically important biomarkers for disease diagnosis. (C) 2009 Elsevier B.V. All rights reserved.
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