期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 7, 页码 1984-1989出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.10.006
关键词
Immunosensors; Carcinoembryonic antigen; Liposome; Carbon nanotube; Screen-printed electrode
Disposable electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) in saliva and serum was developed. Monoclonal anti-CEA antibodies (alpha CEA) were covalently immobilized on polyethyleneimine wrapped multiwalled carbon nanotubes screen-printed electrode. A sandwich immunoassay was performed with CEA and alpha CEA tagged ferrocene carboxylic acid encapsulated liposomes (alpha CEA-FCL). The square wave voltammetry (SWV) was employed to analyze faradic redox responses of the released ferrocene carboxylic acid from the immunoconjugated liposomes on the electrode surface. The magnitude of the SWV peak current was directly related to the concentration of CEA. The calibration curve for CEA concentration was in the range of 5 x 10(-12) to 5 x 10(-7) g mL(-1) with a detection limit of 1 x 10(-12) g mL(-1) (S/N = 3). This method provides a high precise and sensitive determination of CEA in human blood serum and saliva samples. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据