期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 8, 页码 2726-2729出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.12.016
关键词
Electropolymerization of electrochemically or chemically preoxidized catecholamines; Immobilization of glucose oxidase; Amperometric glucose biosensing
类别
资金
- National Natural Science Foundation of China [20675029, 20335020, 90713018]
- Foundations of the Ministry of Education (MOE) of China
- Hunan Provincial Education Department [05009, 05A036]
- State Key Laboratory of Electroanalytical Chemistry
We examine here the electropolymerization of electrochemically or chemically preoxidized catecholamines in glucose oxidase (GOx)-containing neutral solutions to efficiently immobilize the enzyme at Prussian blue-modified Au electrodes for sensitive amperometric biosensing of glucose. The electrochemical quartz crystal microbalance (EQCM) was used to track various electrode-modification processes. The optimized poly(dopamine)-based glucose biosensor displayed a sensitivity of 35 mu A mM(-1) cm(-2) and a limit of detection of 0.3 mu M at 0.7 V vs. SCE, and similar results were obtained at -0.05 V vs. SCE, which are obviously better than those from preoxidation-free conventional electropolymerization. The immobilized Cox retained high enzymatic specific activity, as quantified by UV-vis spectrophotometry and EQCM. L-Noradrenalin could similarly electropolymerize and the resultant enzyme film gave equivalent biosensing characteristics, but the electropolymerization of EP was less efficient and the resultant enzyme film showed notably poorer performance. (C) 2008 Elsevier B.V. All rights reserved.
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