4.8 Article

A multiple-ligand approach to extending the dynamic range of analyte quantification in protein microarrays

期刊

BIOSENSORS & BIOELECTRONICS
卷 24, 期 8, 页码 2458-2464

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.12.030

关键词

Imaging surface plasmon resonance; Biosensor; Affinity microarray; Analyte quantification; Synthetic polypeptide

资金

  1. Swedish Foundation for Strategic Research
  2. European Commission [NMP4-CT-2006-017333]

向作者/读者索取更多资源

This work describes a concept for extending the dynamic range of quantification in an affinity biosensor assay by using a set of ligands with different affinities toward a common analyte. Three synthetic, biotinylated polypeptides capable of binding a model protein analyte with different affinities (10(-9) M <= K-d <= 10(-7) M) were immobilized in a microarray format on a gold slide covered with an oligo(ethylene glycol)-containing alkane thiolate self-assembled monolayer. A five-element affinity array, comprising single-peptide spots as well as spots where peptides were immobilized in mixtures, was realized by means of piezodispensation. Imaging surface plasmon resonance was used to study binding of the analyte to the different spots. The lower limit of analyte quantification was similar to 3 nM and the corresponding upper limit was increased by more than an order of magnitude compared to if only the highest affinity ligand would have been used. Affinity array sensors with multiple ligands for each analyte are particularly interesting for omitting dilution steps and providing highly accurate data in assays where several analytes such as disease biomarkers with extremely variable concentrations are quantified in parallel. (C) 2008 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据