Electrochemical characterisation of a conductive polymer molecularly imprinted with an Amadori compound

Type II diabetes is a disease that is often characterised by elevated levels of advanced glycation end-products (AGEs), e.g. glycated haemoglobin (HbA(1c)), in a patient's bloodstream. This glycation reaction occurs when the carbonyl group of a circulating sugar (glucose) reacts with the amino group of the terminal valine residue of a haemoglobin chain, to form an unstable imine. This compound then undergoes an Amadori rearrangement to form the stable Amadori compound N-(1-deoxy-beta-D-fructopyranose-1-yl)-L-valine (Fru-Val). As an initial approach to fabricating a sensor for the Fru-Val component of HbA(1c), molecular imprints of Fru-Val were made in poly-aminophenylboronic acid (p-APBA), using ammonium persulphate as the initiator, on conductive indium-doped tin oxide (ITO) electrodes (nominal working area 0.5 cm(2)). The affinity of the imprints formed in the p-APBA, for fructose and valine as individual molecules, as well as for the complete template used for imprinting (Fru-Val), was assessed electrochemically, by the use of open circuit potential (Delta E-oc) measurements. These showed that the imprinted materials when challenged with Fru-Val had an open circuit response of similar to 5.0 x 10(-3) V. D-Fructose (10 mM), a component of the template, when introduced into the cell gave a far more significant change in the open circuit potential (Delta E-oc = similar to 2.9 x 10(-3) V) than did a similar concentration Of D-glucose, a non-template carbohydrate (Delta E-oc = similar to 4.0 x 10(-4) V). Non-template structured p-APBA films, made as controls in the absence of Fru-Val, showed negligible response to either D-fructose or D-glucose. Additionally, we have shown that the imprinted films show a progressive reduction in response to sequential additions Of D-fructose, implying the saturation of imprinted sites and a limit to non-specific recognition. (C) 2009 Elsevier B.V. All rights reserved.