期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 1, 页码 66-71出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.03.013
关键词
surface-enhanced Raman scattering; structure-switching signaling aptamer; adenosine
类别
资金
- NSF of China [20435010, 20575020, 20675028, 20605007, 20775023]
- Ministry of Education [NCET-04-0768]
In the present study, we report a novel sensitive method for the detection of adenosine using surface-enhanced Raman scattering (SERS) sensing platform based on a structure-switching aptamer. First, Ag-clad Au colloids film on a polished gold disc is prepared as enhanced substrate and modified with thiolated capture DNA. The formation of an aptamer/DNA duplex of expanded anti-adenosine aptamer and tetramethylrhodamine-labeled DNA (denoted TMR-DNA) is then developed, in which TMR-DNA could also hybridize completely with capture DNA. The introduction of adenosine thus triggers structure switching of the aptamer from aptamer/DNA duplex to aptamer/target complex. As a result, the released TMR-DNA is captured onto the SERS substrate, resulting in an increase of SERS signal. Under optimized assay conditions, a wide linear dynamic range (2.0 x 10(-8) M to 2 x 10(-6) M) was reached with low detection limit (1.0 x 10(-8) M). Moreover, high selectivity, stability and facile regeneration are achieved. The successful test demonstrates the feasibility of the strategy for adenosine assay. (C) 2008 Elsevier B.V. All rights reserved.
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