期刊
BIOSENSORS & BIOELECTRONICS
卷 23, 期 11, 页码 1749-1753出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.02.008
关键词
G-quartet; aptamer; gold nanoparticle; sensor; adenosine triphosphate (ATP); urine sample
This paper describes a colorimetric sensing approach for the determination of adenosine triphosphate (ATP) using aptamer-modified gold nanoparticles (Apt-Au NPs). In the absence of the analytes, the color of the Apt-Au NPs solution changed from wine-red to purple as a result of salt-induced aggregation. Binding of the analytes to the Apt-Au NPs induced folding of the aptamers on the An NP surfaces into four-stranded tetraplex structures (G-quartet) and/or an increase in charge density. As a result, the Apt-Au NPs solution was wine-red in color in the presence of the analytes under high salt conditions. For mixtures of ATP (20.0-100.0 nM), Apt-Au NPs (3.0 nM), 10.0% poly(ethylene glycol), 0.2 mu M TOTO-3, 150.0 mM NaCl, 15.0 mM KCl, and 16.0 mM Tris-HCl (pH 7.4), a linear correlation (R-2 =0.99) existed between the ratio of the extinctions of the Apt-Au NPs at 650 and 520 nm (Ex(650/520)) and the concentration of ATP. The limit of detection for ATP was 10.0 nM. The practicality of this simple, sensitive, specific, and cost-effective approach was demonstrated through the determination of the concentration of adenosine in urine samples. (C) 2008 Elsevier B.V. All rights reserved.
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