期刊
BIOSENSORS & BIOELECTRONICS
卷 23, 期 7, 页码 1216-1219出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.10.021
关键词
catalysts; enzymes; molecularly imprinted polymers (MIPs); thermal methods
To amplify the heat-signal generated by MIP catalyzed solvolysis of phenylacetate the reaction has been combined for the first time in a reactor with the subsequent oxidation by immobilized tyrosinase. The polymer cleaves the substrate and the released phenol is afterwards converted to o-benzoquinone by the tyrosinase. The separated and sequentially coupled reactions are characterized by the heat generated in a thermistor. The sequential substrate conversion results in a combined heat generation which results a five times higher signal than compared to the polymer alone. (c) 2007 Elsevier B.V. All rights reserved.
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