期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 4, 页码 1036-1038出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.06.049
关键词
Immobilisation; Antibody Fab' fragment; Protein repellent polymer; Immunoassay; Surface plasmon resonance; Imprinted self-assembled monolayer
类别
资金
- EC [IST2001-33326]
- Finnish Funding Agency for Technology and Innovation, TEKES [40051/06]
- VrT
Recombinant anti-morphine Fab' fragments have been immobilised on gold by covalent attachment through the free thiol groups of the fragment. The antibody fragments were intercalated with a non-ionic hydrophilic polymer in order to suppress non-specific binding of interfering substances. The antibodies are oriented on the surface due to the thiol groups of the antibody and the layer shows a high response to antigen. Non-specific binding of bovine serum albumin is moreover very low because of the repellent polymer. Synthetic receptors composed of an imprinted self-assembled monolayer made from lipoates and the template, morphine, exhibit the same binding response to the antigen, morphine as the site-specific oriented antibody monolayer. A similar binding curve could be obtained as that for binding of morphine to an antibody Fab' fragment/polymer layer - indicating that synthetic receptors produced are comparable to those of antibody layers. Concentrations down to 0.1 ng/ml have been measured with surface plasmon resonance. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据