期刊
BIOSENSORS & BIOELECTRONICS
卷 23, 期 7, 页码 965-970出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.09.019
关键词
impedimetric aptasensor; Au nanoparticles; thrombin; aptamer
Here, we report a sensitive amplified electrochemical impedimetric aptasensor for thrombin, a kind of serine protease that plays important role in thrombosis and haemostasis. For improving detection sensitivity, a sandwich sensing platform is fabricated, in which the thiolated aptamers are firstly immobilized on a gold substrate to capture the thrombin molecules, and then the aptamer functionalized Au nanoparticles (AuNPs) are used to amplify the impedimetric signals. Such designed aptamer/thrombin/AuNPs sensing system could not only improve the detection sensitivity compared to the reported impedimetric aptasensors but also provide a promising signal amplified model for aptamer-based protein detection. In this paper, we realize a sensitive detection limit of 0.02 nM, with a linear range of 0.05-18 nM. Meanwhile, the effect of 6-mercaptohexanol (MCH) and 2-mercaptoethanol (MCE) on the modification of the electrode is investigated. (C) 2007 Elsevier B.V. All rights reserved.
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