期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 4, 页码 928-933出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.07.053
关键词
Thiophene conducting polymer; DNA sensor; Gene sensor; EIS
类别
资金
- Auckland UniServices
- The University of Auckland, New Zealand
The gene-sensing properties of sensor films made of a terthiophene-conducting polymer, poly(3-((2':2 '', 5:2'-terthiophene)-3 ''-yl)acrylic acid) (PTAA), were evaluated using electrochemical impedance spectroscopy for films in their reduced and oxidised states with and without the Fe(CN)(6)(3-/4-) redox probe (RP) in dilute tris-EDTA buffer. Porous films of PTAA were prepared and attached to an oligonucleotide sequence specific to the Salmonella virulence gene lnvA. These films could be described with a dual transmission line model in which the polymer conductivity was increased as a consequence of surface binding of complementary DNA. The effect is analogous to that reported for silicon nanowires and field-effect transistors in dilute electrolyte modified by charge exchange across the polymer-electrolyte interface. As a result, gene sensing could be conveniently observed as a change in the impedance phase angle at a fixed frequency. (C) 2008 Elsevier B.V. All rights reserved.
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