期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 4, 页码 1027-1030出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.06.039
关键词
Bioelectric recognition assay; Cell biosensor; Cucumber green mottle mosaic virus; Tobacco rattle virus; Cell biosensor; Membrane-engineering
类别
资金
- Ministry of Industry, Commerce and Tourism
- European Social Fund
- National Resources-O.P. Education II
- Greek-Bulgarian joint Research and Technology Programme 4.3.6.1
A novel concept for the assay of viral antigens is described. The methodological approach is based on a membrane-engineering process involving the electroinsertion of virus-specific antibodies in the membranes of fibroblast cells. As a representative example, Vero fibroblasts were engineered with antibodies against Cucumber mosaic virus (CMV) and used for the construction of an ultra-sensitive miniature cell biosensor system. The attachment of a homologous virus triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the bioelectric recognition assay (BERA). No change in the membrane potential was observed upon cell contact with the heterologous cucumber green mottle mosaic virus (CGMMV). Fluorescence microscopy observations showed that attachment of CMV particles to membrane-engineered cells was associated with membrane hyperpolarization and increased [Ca2+](cyt). In an additional field-based application, we were able to detect CMV-infected tobacco plants at an essentially 100% level of accuracy. (C) 2008 Elsevier B.V. All rights reserved.
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