期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 4, 页码 558-565出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.06.019
关键词
DNA detection; Magnetic bead; Quantum dots; Biotin-streptavidin; Biotinlyted linker
类别
资金
- National Science Council of ROC [NSC96-2221-E007-128-MY3]
This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis. (C) 2008 Elsevier B.V. All rights reserved.
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