期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 78, 期 12, 页码 2128-2133出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2014.946398
关键词
molecular breeding; Phanerochaete sordida YK-624; glyoxal oxidase; lignin degradation
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [21248023, 24248030]
- Grants-in-Aid for Scientific Research [21248023] Funding Source: KAKEN
Glyoxal oxidase (GLOX) is a source of the extracellular H2O2 required for the oxidation reactions catalyzed by the ligninolytic peroxidases. In the present study, the GLOX-encoding gene (glx) of Phanerochaete chrysosporium was cloned, and bee2 promoter of P. sordida YK-624 was used to drive the expression of glx. The expression plasmid was transformed into a P. sordida YK-624 uracil auxotrophic mutant (strain UV-64), and 16 clones were obtained as GLOX-introducing transformants. These transformants showed higher GLOX activities than wild-type P. sordida YK-624 and control transformants harboring marker plasmid. RT-PCR analysis indicated that the increased GLOX activity was associated with elevated recombinant glx expression. Moreover, these transformants showed higher ligninolytic activity than control transformants. These results suggest that the ligninolytic properties of white-rot fungi can be improved by recombinant expression of glx.
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