期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 77, 期 1, 页码 1-9出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.120759
关键词
benzylisoquinoline alkaloid biosynthesis; functional differentiation; photoautotrophic culture; secondary metabolism; somaclonal variation
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Japan Society for the Promotion of Science
- Future Program Grant [JSPS-RFTF00L01606]
- NEDO
- Grants-in-Aid for Scientific Research [25108713, 21248013] Funding Source: KAKEN
Plant cell cultures are widely used in the micropropagation of clonal plants, especially virus-free plants, and in the production of useful metabolites such as paclitaxel. On the other hand, the use of plant cell cultures for the more basic characterization of plant functions is rather limited due to the difficulties associated with functional differentiation in cell cultures. In this review, I overview our experience with functionally differentiated cultured plant cells and their characteristics, especially with regard to photoautotrophism and secondary metabolism. I emphasize the high potential of functionally differentiated cell cultures, as well as some of the pitfalls, in the characterization of plant functions and biotechnological applications.
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