期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 77, 期 8, 页码 1788-1791出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.130357
关键词
SUMOylation; deSUMOylation; SENP; medaka fish (Oryzias latipes)
类别
资金
- Japan Society for the Promotion of Science [23616004]
- Ministry of Education, Culture, Sports, Science and Technology [23131519]
- Grants-in-Aid for Scientific Research [23616004, 23370030, 23131519] Funding Source: KAKEN
In this study, we cloned the catalytic domain of the Oryzias latipes sentrin/SUMO-specific protease 1 (OlSENP1-CD) gene and produced the recombinant OlSENP1-CD protein in Escherichia coli. Experimental procedures designed to reveal the ability of the recombinant protein to show deSUMOylating activity in vitro should be helpful in future studies of other SENPs and the SUMO pathway.
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