期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 76, 期 12, 页码 2255-2260出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.120521
关键词
avian infectious bronchitis virus; comparation; dynamic distribution; quantitative real-time reverse transcription-polymerase chain; reaction
类别
资金
- Foundation for Chinese National Programs for High Technology Research and Development [2011AA10A209]
- Modern Agro-industry Technology Research System [CARS-41-K09]
- Natural Science Foundation of China [30972201]
Avian infectious bronchitis is an acute, highly contagious disease of chickens. To study the differences of dynamic distribution between nephropathogenic infectious bronchitis virus (IBV) strains such as SAIBK and other strains (the M41 and H120 strains), relative quantitative real-time reverse transcription-polymerase chain reaction was developed by housekeeping gene selection. Glyceraldehyde-3-phosphate dehydrogenase and Ubiquitin were chosen for normalization in this experimental set. Then nine tissues, the trachea, thymus, liver, spleen, lungs, kidney, pancreas, proventriculus, and bursa of Fabricius, were analyzed and compared to determine the tropism of IBV infection. In this research, the kidney and the lung were established of the most sensitive organs in IBV infection. The pancreas and the liver are candidates for antigen detection. The trachea and the spleen can be used as references for histological diagnosis, but they are not suitable for antigen detection; proventriculus might be an important target in IBV infection; the thymus and the bursa of Fabricius were not sensitive organs in IBV infection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据