期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 76, 期 6, 页码 1248-1251出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.120226
关键词
mRNA processing; inhibitor; luciferase reporter; RNA-fluorescence in situ hybridyzation (FISH); reverse transcription-polymerase chain reaction (RT-PCR)
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资金
- Central Miso Research Institute
- IST in research for adaptable and seamless technology transfer program through target-driven R&D, exploratory research stage
- Kieikai Research Foundation
- Grants-in-Aid for Scientific Research [23880015] Funding Source: KAKEN
A number of proteins complete mRNA processing in the nucleus, thus, inhibitor of mRNA processing is worth finding to analyze the mechanism of mRNA maturation in detail. Here, we established a monitoring system for mRNA processing using a test compound, spliceostatin A (SSA), which inhibits mRNA splicing. This system should serve to facilitate the discovery of novel compounds from natural resources that inhibit mRNA processing.
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