期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 75, 期 8, 页码 1472-1480出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.110137
关键词
Ecklonia stolonifera; Eisenia bicyclis; protein tyrosine phosphatase 1B; alpha-glucosidase; phlorotannin
类别
资金
- Blue-Bio Industry RIC at Dong-Eui University of ITEP under MKE
- Blue-Bio Industry RIC at Dong-Eui University of ITEP under Busan City
- Ministry for Food, Agriculture, Forestry and Fisheries in Republic of Korea
- Ministry of Knowledge Economy (MKE), Republic of Korea [B0010792] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The present work investigates protein tyrosine phosphatase 1B (PTP1B) and the alpha-glucosidase inhibitory activities of two edible brown algae, Ecklonia stolonifera and Eisenia bicyclis, as well as in their isolated phlorotannins. Since the individual extracts and fractions showed significant inhibitory activities, column chromatography was performed to isolate six phlorotannins, phloroglucinol (1), dioxinodehydroeckol (2), eckol (3), phlorofurofucoeckol-A (4), dieckol (5), and 7-phloroeckol (6). Phlorotannins 3-6 were potent and noncompetitive PTP1B inhibitors with IC50 values ranging from 0.56 to 2.64 mu M; 4-6 exhibited the most potent alpha-glucosidase inhibition with IC50 values ranging from 1.37 to 16.13 mu M. Interestingly, 4 and 6 were noncompetitive, while 5 exhibited competitive inhibition in an alpha-glucosidase assay. E. stolonifera and E. bicyclis as well as their isolated phlorotannins therefore possessed marked PTP1B and alpha-glucosidase inhibitory activities; this could lead to opportunities in the development of therapeutic agents to control the postprandial blood glucose level and thereby prevent diabetic complications.
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