期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 8, 页码 1564-1571出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100135
关键词
Clostridium petfringens; toxin gene regulation; Tex; RNA-binding protein
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology [16380055]
- Grants-in-Aid for Scientific Research [16380055] Funding Source: KAKEN
Tex was originally identified in Bordetella pertussis, where it serves as a transcriptional regulator of toxin genes. However, the Tex of Streptococcus pneumoniae has no regulatory function in the expression of the pneumococcal major toxin pneumolysin. Here, we identified the CPE2168 gene as Tex in Clostridium perfringens, and examined the roles of Tex in toxin gene expression. We found that the deletion mutant for Tex does not affect growth, but the mRNA levels of three hyaluronidase genes (nagH, nagJ, and nagL) and an exo-sialidase (nanJ) were reduced to less than 50% as compared to the parent strain, C. perfringens strain 13. On the other hand, Tex did not affect the expression of proteases, enterotoxins, hemolysins, either of two hyaluronidase genes (nag! and nagK), an exo-sialidase (nanI), or adhesins. Moreover, purified Tex bound to the 5'-portion of target gene mRNAs. Based on these results, we propose that Tex positively regulates the gene expression of a set of toxin genes in C. perfringens.
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