期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 7, 页码 1403-1407出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100071
关键词
fungal denitrification; copper-containing nitrite reductase; flavohemoglobin; cytochrome P450 nitric oxide reductase; suppression subtraction hybridization
类别
资金
- Japan Society for the Promotion of Science [20248009]
- Research and Development Program for New Bio-industry Initiatives of Japan
- Grants-in-Aid for Scientific Research [20248009] Funding Source: KAKEN
The occurrence of denitrification and nitrate respiration among eukaryotes has been established during the last few decades. However, denitrification-related eukaryotic genes have been isolated from only a few fungi, and eukaryotic denitrification (or nitrate respiration) is still inadequately understood. In this study, we identified genes that were up-regulated under denitrifying conditions in the fungus Cylindrocarpon tonkinense using the suppression subtraction hybridization technique, and the expression patterns of these genes were characterized by Northern analysis. We identified copper-containing nitrite reductase, cytochrome P450 nitric oxide reductase, flavohemoglobin (Fhb), and formate/nitrite transporter homolog genes as possibly involved in fungal denitrification. Our results concerning the involvement of Fhb and formate/nitrite transporter perhaps provide new insight into the fungal denitrification system.
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