期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 74, 期 8, 页码 1722-1724出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.100299
关键词
basidiomycete; carbohydrate esterase; Coprinopsis cinerea
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Green Biomass Research
cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, alpha-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-beta-1,4-xylanase.
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